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Indexed by:期刊论文

Date of Publication:2009-05-01

Journal:SCIENCE IN CHINA SERIES B-CHEMISTRY

Included Journals:SCIE、EI

Volume:52

Issue:5

Page Number:615-620

ISSN No.:1006-9291

Key Words:catechol 1,2-dioxygenase; cell extracts; characterization; Sphingomonas xenophaga

Abstract:Sphingomonas xenophaga QYY, capable of growing significantly on more than ten kinds of aromatic compounds as sole carbon source, was used to study characterization of catechol 1,2-dioxygenase (C12O) in cell extracts. Characterization of the crude C12O showed that the maximum activity was obtained at 40-70A degrees C and pH 7.8-8.8. Metal ions had different influences on the activity of crude C12O. It was suggested that strain QYY possessed an inducible and ferric-dependent C12O. Kinetic studies showed that the value of V (max) and K (m) was 0.25 mu mol catechol/L/mg protein/min and 52.85 mu mol/L, respectively. In addition, the partial purification of C12O was achieved by a HiTrap Q Sepharose column chromatography.