Hits:

Indexed by:期刊论文

Date of Publication:2009-07-01

Journal:JOURNAL OF ENVIRONMENTAL SCIENCES

Included Journals:SCIE、EI、PubMed、ISTIC、CSCD

Volume:21

Issue:6

Page Number:821-826

ISSN No.:1001-0742

Key Words:phenolic compounds; high salt; phenol hydroxylase; ribosomal intergenic spacer analysis; amplified functional DNA restriction analysis

Abstract:The changes of microbial community structures and functional genes during the biodegradation of single phenol and phenol plus p-cresol under high salt condition were explored. It was found that the phenol-fed system (PFS) exhibited stronger degrading abilities and more stable biomass than that of the phenol plus p-cresol-fed system (PCFS). The microbial community structures were revealed by a modem DNA fingerprint technique, ribosomal intergenic spacer analysis (RISA). The results indicated that the microbial community of PFS changed obviously when gradually increased phenol concentration, while PCFS showed a little change. 16S rRNA sequence analysis of the major bands showed that Alcanivorax sp. genus was predominant species during phenolic compounds degradation. Furthermore, amplified functional DNA restriction analysis (AFDRA) on phenol hydroxylase genes showed that the fingerprints were substantially different in the two systems, and the fingerprints were not the same during the different operational periods.