Release Time:2019-03-10  Hits:

Indexed by: Journal Article

Date of Publication: 2008-07-01

Journal: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Included Journals: EI、SCIE

Volume: 24

Issue: 7

Page Number: 1147-1152

ISSN: 0959-3993

Key Words: Sphingomonas xenophaga; 1-aminoanthraquinone-2-sulfonic acid; anthraquinone dye intermediate; decolorization; degradation

Abstract: Sphingomonas xenophaga QYY from sludge samples could effectively decolorize 1-aminoanthraquinone-2-sulfonic acid (ASA-2), one kind of anthraquinone dye intermediate, under aerobic conditions. More than 98% of ASA-2 could be removed within 120 h at the dye concentration from 200 mg l(-1) to 1,000 mg l(-1) due to oxidative degradation. The strain converted ASA-2 to 2-(2'-hydroxy-3'-amino-4'-sulfo-benzoyl)-benzoic acid, 2-(2'-amino-3'-sulfo-6'-hydroxy-benzoyl)-benzoic acid, o-phthalic acid and 2-amino-3-hydroxy-benzenesulfonic acid, which were identified using HPLC-MS and NMR. A possible initial decolorization pathway was proposed according to these metabolites. The decolorization of ASA-2 by cells in the basal salt medium was induced by ASA-2, and was due to soluble cytosolic enzymes. Combined initial decolorization pathway and the analysis of decolorization enzyme(s), the major enzyme responsible for ASA-2 decolorization was a NADH-dependent oxygenase.