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Indexed by:期刊论文

Date of Publication:2008-07-01

Journal:WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Included Journals:SCIE、EI

Volume:24

Issue:7

Page Number:1147-1152

ISSN No.:0959-3993

Key Words:Sphingomonas xenophaga; 1-aminoanthraquinone-2-sulfonic acid; anthraquinone dye intermediate; decolorization; degradation

Abstract:Sphingomonas xenophaga QYY from sludge samples could effectively decolorize 1-aminoanthraquinone-2-sulfonic acid (ASA-2), one kind of anthraquinone dye intermediate, under aerobic conditions. More than 98% of ASA-2 could be removed within 120 h at the dye concentration from 200 mg l(-1) to 1,000 mg l(-1) due to oxidative degradation. The strain converted ASA-2 to 2-(2'-hydroxy-3'-amino-4'-sulfo-benzoyl)-benzoic acid, 2-(2'-amino-3'-sulfo-6'-hydroxy-benzoyl)-benzoic acid, o-phthalic acid and 2-amino-3-hydroxy-benzenesulfonic acid, which were identified using HPLC-MS and NMR. A possible initial decolorization pathway was proposed according to these metabolites. The decolorization of ASA-2 by cells in the basal salt medium was induced by ASA-2, and was due to soluble cytosolic enzymes. Combined initial decolorization pathway and the analysis of decolorization enzyme(s), the major enzyme responsible for ASA-2 decolorization was a NADH-dependent oxygenase.