点击次数：

论文类型：期刊论文

发表时间：2015-03-01

发表刊物：BIORESOURCE TECHNOLOGY

收录刊物：SCIE、EI、PubMed、ESI高被引论文

卷号：179

页面范围：436-443

ISSN号：0960-8524

关键字：Coking wastewater treatment plants; Illumina high-throughput sequencing; Bacterial community composition; Core population; Nitrification

摘要：In this study, Illumina high-throughput sequencing was used to reveal the community structures of nine coking wastewater treatment plants (CWWTPs) in China for the first time. The sludge systems exhibited a similar community composition at each taxonomic level. Compared to previous studies, some of the core genera in municipal wastewater treatment plants such as Zoogloea, Prosthecobacter and Gp6 were detected as minor species. Thiobacillus (20.83%), Comamonas (6.58%), Thauera (4.02%), Azoarcus (7.78%) and Rhodoplanes (1.42%) were the dominant genera shared by at least six CWWTPs. The percentages of autotrophic ammonia-oxidizing bacteria and nitrite-oxidizing bacteria were unexpectedly low, which were verified by both real-time PCR and fluorescence in situ hybridization analyses. Hierarchical clustering and canonical correspondence analysis indicated that operation mode, flow rate and temperature might be the key factors in community formation. This study provides new insights into our understanding of microbial community compositions and structures of CWWTPs. (C) 2014 Elsevier Ltd. All rights reserved.