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论文类型：期刊论文

发表时间：2014-03-01

发表刊物：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录刊物：SCIE、EI、PubMed、Scopus

卷号：172

期号：6

页面范围：3194-3206

ISSN号：0273-2289

关键字：Indirubin; Tryptophan; 2-oxindole; Naphthalene dioxygenase; Response surface methodology

摘要：Indirubin, a red isomer of indigo, can be used for the treatment of various chronic diseases. However, the microbial production of indirubin did not receive much attention probably due to its low yield compared with indigo. In this study, the recombinant Escherichia coli containing the naphthalene dioxygenase (NDO) genes from Comamonas sp. MQ was used to produce indirubin from tryptophan. To enhance the production of indirubin, the induction conditions for NDO expression were optimized. The optimal induction conditions were carried out with 0.5 mM isopropyl-beta-D-thiogalactopyranoside at 30 degrees C when cells were grown to OD600 approximate to 1.20. Subsequently, the effects of medium composition on indirubin production were investigated by response surface methodology, and 9.37 +/- 1.01 mg/l indirubin was produced from 3.28 g/l tryptophan. Meanwhile, the indirubin production was further improved by adding 2-oxindole and isatin to the tryptophan medium after induction. About 57.98 +/- 2.62 mg/l indirubin was obtained by the addition of 500 mg/l 2-oxindole after 1-h induction, which was approximately 6.2-fold to that without additional 2-oxindole. The present study provided a possible way to improve the production of indirubin and should lay the foundation for the application of microbial indirubin production.