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论文类型：期刊论文

发表时间：2013-06-01

发表刊物：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录刊物：SCIE、EI、Scopus

卷号：97

期号：11

页面范围：5097-5103

ISSN号：0175-7598

关键字：Biotransformation; Phenol hydroxylase; Catechol; Toluene; Benzene

摘要：Phenol hydroxylase gene engineered microorganism (PHIND) was used to synthesize catechols from benzene and toluene by successive hydroxylation reaction. HPLC-MS and H-1 NMR analysis proved that the products of biotransformation were the corresponding catechols via the intermediate production of phenols. It was indicated that the main products of toluene oxidation were o-cresol and p-cresol. 3-Methylcatechol was the predominant product for m-cresol biotransformation. Formation rate of catechol (25 mu M/min/g cell dry weight) was 1.43-fold higher than that of methylcatechols. It was suggested that phenol hydroxylase could be successfully used to transform both benzene and toluene to catechols by successive hydroxylation.