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论文类型：期刊论文

发表时间：2013-06-01

发表刊物：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录刊物：Scopus、EI、SCIE

卷号：170

期号：4

页面范围：951-961

ISSN号：0273-2289

关键字：Phenol hydroxylase; Chloro-substituted indoles; Biotransformation; Response surface methodology

摘要：Recombinant Escherichia coli cells expressing phenol hydroxylase (designated as strain PHIND) were used to synthesize chloro-substituted indigoids by the transformation of indoles. The optimal conditions for the biotransformation of 4- and 7-chloroindole were determined by response surface methodology. Biotransformation kinetic assays revealed that strain PHIND showed high catalytic efficiency for 4- and 7-chloroindole. The formation rate of 7,7'-dichloroindigo (1.35 unit/mg cell dry weight) by strain PHIND was 1.14-fold higher than that of 4,4'-dichloroindigo. The intermediates of 7-chloroindole biotransformation were identified by high-performance liquid chromatography-mass spectroscopy, and the biotransformation mechanism was also proposed. These results suggested that there was a potential application of strain PHIND in the biotransformation of chloro-substituted indoles to valuable indigoids.