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论文类型：期刊论文

发表时间：2012-12-01

发表刊物：ENVIRONMENTAL TECHNOLOGY

收录刊物：SCIE、PubMed、Scopus

卷号：33

期号：23

页面范围：2603-2609

ISSN号：0959-3330

关键字：Streptomyces; alkali lignin; biodegradation; co-culture; GC-MS analysis

摘要：Biodegradation and bioconversion of lignin are the result of the combined action of fungi, bacteria and actinomycetes. Through screening from forest soil, two novel isolated actinomycete strains were identified as Streptomyces spp. strains F-6 and F-7 by their morphology, cultural characteristics and high homology to the 16S rRNA gene. Both strains possessed laccase and manganese peroxidase activities. Laccase activity produced by strain F-6 was up to 935.4Ug(-1) dry cell weight. More than 50% of alkali lignin was removed by strains F-6 and F-7 in 12 days of incubation. GC-MS analysis of the biodegraded products showed strain F-6 converted lignin into phenol and broken phenol compounds. The two strains could co-culture with white-rot fungus, and the combined actinonycete-fungus system decomposed alkali lignin effectively.