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论文类型：期刊论文

发表时间：2009-05-01

发表刊物：SCIENCE IN CHINA SERIES B-CHEMISTRY

收录刊物：SCIE、EI

卷号：52

期号：5

页面范围：615-620

ISSN号：1006-9291

关键字：catechol 1,2-dioxygenase; cell extracts; characterization; Sphingomonas xenophaga

摘要：Sphingomonas xenophaga QYY, capable of growing significantly on more than ten kinds of aromatic compounds as sole carbon source, was used to study characterization of catechol 1,2-dioxygenase (C12O) in cell extracts. Characterization of the crude C12O showed that the maximum activity was obtained at 40-70A degrees C and pH 7.8-8.8. Metal ions had different influences on the activity of crude C12O. It was suggested that strain QYY possessed an inducible and ferric-dependent C12O. Kinetic studies showed that the value of V (max) and K (m) was 0.25 mu mol catechol/L/mg protein/min and 52.85 mu mol/L, respectively. In addition, the partial purification of C12O was achieved by a HiTrap Q Sepharose column chromatography.