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论文类型：期刊论文

发表时间：2009-04-01

发表刊物：JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLOGY

收录刊物：SCIE、EI、Scopus

卷号：16

期号：2

页面范围：230-235

ISSN号：1005-9784

关键字：Micrococcus; 16S rDNA; nitrobenzene; degradation; salt-tolerant

摘要：A novel strain of Micrococcus sp. DUT_AHX, which was isolated from the sludge of a nitrobenzene (NB)-manufacturing plant and could utilize NB as the sole carbon source, was identified on the basis of physiological and biochemical tests and 16S ribosomal DNA (rDNA) sequence analysis. It can grow at the temperature up to 40 degrees C or in the presence of NaCl concentration up to 12 g/L in Luria-Bertani (LB) medium. The optimal degradation conditions are as follows: temperature 37 degrees C, pH 7.0, and shaking speed 150 r/min. The strain involves a partial reductive pathway due to the release of ammonia and can also utilize 2-aminophenol as the sole carbon source. Furthermore, the enzyme activity tests show that crude extracts of NB-grown strain DUT_AHX mainly contain 2-aminophenol 1, 6-dioxygenase activity. The exploitation of salt-tolerant bacteria will be a remarkable improvement in NB bioremediation and wastewater treatment at high salinity and high temperature.