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个人信息Personal Information
教授
博士生导师
硕士生导师
性别:男
毕业院校:大连工学院
学位:硕士
所在单位:环境学院
电子邮箱:zjiti@dlut.edu.cn
Quinone-mediated decolorization of sulfonated azo dyes by cells and cell extracts from Sphingomonas xenophaga
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论文类型:期刊论文
发表时间:2009-07-01
发表刊物:JOURNAL OF ENVIRONMENTAL SCIENCES
收录刊物:SCIE、EI、PubMed、ISTIC、CSCD、Scopus
卷号:21
期号:4
页面范围:503-508
ISSN号:1001-0742
关键字:redox mediator; sulfonated azo dyes; Sphingomonas xenophaga; reductase
摘要:The effects of various quinone compounds on the decolorization rates of sulfonated azo dyes by Sphingomonas xenophaga QYY were investigated. The results showed that anthraquinone-2-sulfonate (AQS) was the most effective redox mediator and AQS reduction was the rate-limited step of AQS-mediated decolorization of sulfonated azo dyes. Based on AQS biological toxicity tests, it was assumed that AQS might enter the cells and kill them. In the cytoplasmic extracts from strain QYY, AQS more effectively increased decolorization rates of sulfonated azo dyes than other quinone compounds. In addition, we found a NADH/FMN-dependent AQS reductase using nondenaturing polyacrylamide gel electrophoresis (Native-PAGE).