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论文类型：会议论文

发表时间：2016-01-01

收录刊物：CPCI-S

卷号：88

页面范围：2021-2026

关键字：Immnuoisolation; Perfusion; NSCs; Expansion; Co-culture

摘要：Considering the immnuoisolation from the neural stem cells (NSCs) and endothelial progenitor cells (EPCs), the requirements of the carrier, shear tolerance and other factors, this study designed an immunoisolation perfusion co-culture bioreactor system, by dynamic perfusion culture environment of its upper and lower bi-levels and the immnuoisolation of intermediate polycarbonate membrane, combining the measure of embedding NSCs and EPCs with calcium alginate/gelatin microbeads, integrating hypoxic environment effectively to achieve the expansion of NSCs. The results show that the microbeads are more evenly distributed under dynamic perfusion conditions, the velocity of medium is slightly larger near polycarbonate membrane and the culture chamber wall surface, and has more balanced distribution of other regions, static pressure and dynamic pressure of the culture chamber are generally evenly distributed. Studies showed that the expansion of NSCs under perfusion conditions, is able to meet the mass transfer and provide a stable environment to the neural stem cell expansion.