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个人信息Personal Information
教授
博士生导师
硕士生导师
性别:男
毕业院校:大连理工大学
学位:博士
所在单位:化工学院
学科:化学工程. 生物医学工程
办公地点:1. 西部校区D段楼305
2. 环境生命楼3-403
联系方式:Email: liutq@dlut.edu.cn QQ: 343028655
电子邮箱:liutq@dlut.edu.cn
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Vitrification of Corneal Endothelial Cells in a Monolayer
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论文类型:期刊论文
发表时间:2008-12-01
发表刊物:JOURNAL OF BIOSCIENCE AND BIOENGINEERING
收录刊物:SCIE、EI、PubMed、Scopus
卷号:106
期号:6
页面范围:610-613
ISSN号:1389-1723
关键字:corneal endothelial cells; vitrification; cryoprotectant; cell monolayer
摘要:An in vitro model was developed for bovine natural corneal endothelia. The cells were cultured to a confluent monolayer and vitrified using 25% (w/w) 1,2-propanediol-35% (w/w) trehalose as cryoprotective agents. Approximately, 61.3% of the cells were viable using the protocol.