论文类型：会议论文
收录刊物：Scopus、SCIE、CPCI-S、EI
卷号：7909
关键字：Ag/SiO2 nanocomposites; metal-enhanced fluorescence; time-gated bioimaging; Eu chelate; single particle detection
摘要：We report silver nanostructure-enhanced fluorescence of a europium (Eu) chelate, BHHCT-Eu-DPBT, which was covalently bound in Ag/SiO2 nanocomposites. This design enhances the europium signal intensity by more than one order of magnitude, and accelerates the decay time from 0.3 ms down to 60 microseconds, at low excitation conditions. These nanocomposites were bright enough to be observed in time-gated fluorescence microscopy under 365 nm LED excitation. The increased brightness and reduced lifetime of such fluorescent core-shell nanocomposites will enhance their applicability for ultrasensitive bioassays and bioimaging, especially with time-gating.