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发表时间：2016-01-01

发表刊物：中国饲料

期号：8

页面范围：25-28,42

ISSN号：1004-3314

摘要：Solid-state culture of Aspergillus niger and Saccharomyces cerevisiae was studied by using corn bran as main material.Single-factor and orthogonal experiment were used to optimize the nutritional and environmental conditions for improving the activity of protease,cellulase and the number of living cells.The optimized cultivation conditions for As-pergillus niger were as follows:urea content 0.5%(P < 0.05),water content 60%,wheat bran adding level 15%(P <0.05),inoculation amount 20%,cultivation period 7 days.When co-culture of two strains was conducted,the ratio of As-pergillus niger to Saccharomyces cerevisiae was 3∶1,and co-culture time was 1 day.Under the above conditions,the cellu-lase activity reached to 622.11U/g,the protease activity achieved 605.55U/g,and the total number of living probiotic cells reached to more than ten billion per gram.

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