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论文类型：期刊论文

发表时间：2022-06-30

发表刊物：中国科学 化学

所属单位：生物工程学院

卷号：45

期号：5

页面范围：558-563

ISSN号：1674-7224

摘要：Salting-out extraction is an efficient method to separate the
   hydrophilic molecules with low-molecular weight from fermentation broth,
   and its process is discontinuous at present. In this study a continuous
   salting-out extraction system is constructed which is composed of a
   micro-channel mixer and a stationer, and the effect of mixing time on
   phase formation and 2,3-butanediol partition behavior was explored. The
   experiments was carried out with a salting-out system formed by 24%
   ethanol (w/w) and 25% K_2HPO_4 (w/w), and the results showed that mixing
   time has no obvious effect on the recovery of 2,3-butanediol, but
   affects the phase ratio obviously in the mixing time of 9 to 60 s. The
   least phase ratio was obtained at the mixing time of 20.7 s, and the
   recovery was above 96% after the 8 h standing under continuous mixing
   condition. When the retention time in micro-channel mixer and stationer
   was 20.7 s and 25 min, respectively, the concentration of 2,3-butanediol
   was 78 g/L in upper phase and 5.63 g/L in the bottom which has little
   fluctuation during the process, and the partition coefficient and
   recovery was 13.85 and 96.2%, respectively. The results verified the
   feasibility of continuous salting-out extraction of 2,3-butanediol.

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