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论文类型：期刊论文

发表时间：2013-12-15

发表刊物：FOOD CHEMISTRY

收录刊物：SCIE、EI、PubMed

卷号：141

期号：4

页面范围：3317-3322

ISSN号：0308-8146

关键字：Feruloylated lysophospholipids; Ethyl ferulate; Lipase; Phosphatidylcholine; Enzymatic modification

摘要：Feruloylated lysophospholipids (FLPs) were firstly synthesized from phosphatidylcholine (PC) and ethyl ferulate (EF) using lipase-catalysed interesterification in selected solvents at controlled water content. Kinds of lipases and single solvents were screened. Novozym 435 and toluene were found to be the suitable biocatalyst and solvent, respectively. Then tert-butanol, n-butanol, chloroform, isopropanol, acetone and DMSO were respectively added into toluene in order to increase conversion of products. The results showed that toluene/chloroform could significantly increase the conversion and the optimal combination of toluene and chloroform was 90:10 (v/v). The optimal conditions generated for FLPs production were a substrate molar ratio of 5:1 (PC/EF), a PC's hydrolytic time of 1.5 h, an enzyme load of 60 mg/ml, a solvent dosage of 5 ml and a molecular sieves concentration (4 angstrom) of 100 mg/ml. Under these conditions, 40.51% of EF can be converted to FLPs, which were identified by TLC and HPLC-MS. (C) 2013 Elsevier Ltd. All rights reserved.