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论文类型：期刊论文

发表时间：2010-09-01

发表刊物：BIOTECHNOLOGY LETTERS

收录刊物：SCIE、EI、PubMed

卷号：32

期号：9

页面范围：1245-1250

ISSN号：0141-5492

关键字：Dielectric barrier discharge; Enzyme activity; Fermentation; Klebsiella pneumoniae; Metabolic flux; 1,3-Propanediol

摘要：Dielectric barrier discharge plasma was used to generate a stable strain of Klebsiella pneumoniae (designated to as Kp-M2) with improved 1,3-propanediol production. The specific activities of glycerol dehydrogenase, glycerol dehydatase and 1,3-propanediol oxidoreductase in the crude cell extract increased from 0.11, 9.2 and 0.15 U mg(-1), respectively, for wild type to 0.67, 14.4 and 1.6 U mg(-1) for Kp-M2. The glycerol flux of Kp-M2 was redistributed with the flux to the reductive pathway being increased by 20% in batch fermentation. The final 1,3-propanediol concentrations achieved by Kp-M2 in batch and fed-batch fermentations were 19.9 and 76.7 g l(-1), respectively, which were higher than those of wild type (16.2 and 49.2 g l(-1)). The results suggested that dielectric barrier discharge plasma could be used as an effective approach to improve 1,3-propanediol production in K. pneumoniae.