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Indexed by:期刊论文
Date of Publication:2018-06-01
Journal:JOURNAL OF MOLECULAR LIQUIDS
Included Journals:SCIE、EI
Volume:259
Page Number:398-410
ISSN No.:0167-7322
Key Words:Doxorubicin hydrochloride; Amino acid gemini surfactant; Self-association; Catanionic vesicles; Sustained drug release; Cytotoxicity
Abstract:In this study, we provide an alternative formulation to develop gemini surfactant (GSs)-drug nano-sized catanionic vesicles with multi stimuli-responsiveness, which have great potential applications in the fields of controlled release and drug delivery in chemotherapy. The main aim of this study was to investigate how catanionic vesicles could influence the encapsulation and release of the anticancer drug Doxorubicin (DOX). The DOX was self-associated in an aqueous medium according to reported studies still controversial. We present here the simple and convenient methods, surface tension, conductance and circular dichroism (CD) studies to give direct evidence that DOX was self-associated at the critical concentration about 0.841 mmol L-1. The influence of micelle based on DOX-loaded (DLs) and mixed micelle (C*(alpha(DOX) + alpha(GSs))) catanionic vesicles (DGCs) showed the highly synergistic interaction. The physicochemical properties, molecular docking, drug uptake and release of DOX in in-vitro for DL and DGC were explored. The substantial amounts of DOX could successfully encapsulate into the catanionic vesicles. The important point of DGC vesicles was they could reduce toxicity and revealed better therapeutic effects when the DOX was in high concentration. Moreover, the cell viability of DL-12 vesicles was 44%, and DL-16 vesicles was 70% at 18 mu mol L-1, while at 20.0 mu mol L-1 it was 44% in DGC-12 as alpha(DOX) was 0.6 and 56% in DGC-16 as alpha(DOX) was 0.9. The in-vitro cytotoxicity and fluorescence microscopic images of DL and DGC vesicles on MCF-7 cell lines showed the improved nuclear localization of DOX uptakes. These findings showed the high importance of catanionic vesicles to enhance the free DOX entrance mainly via endocytosis for breast cancer cell. (C) 2018 Elsevier B.V. All rights reserved.
