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论文类型：期刊论文

发表时间：2014-05-21

发表刊物：JOURNAL OF MATERIALS CHEMISTRY B

收录刊物：SCIE、EI、PubMed、Scopus

卷号：2

期号：19

页面范围：2920-2928

ISSN号：2050-750X

摘要：To obtain efficient non-viral vectors, a series of Gemini cationic lipids with carbamate linkers between headgroups and hydrophobic tails were synthesized. They have the hydrocarbon chains of 12, 14, 16 and 18 carbon atoms as tails, designated as G12, G14, G16 and G18, respectively. These Gemini cationic lipids were prepared into cationic liposomes for the study of the physicochemical properties and gene delivery. The DNA-bonding ability of these Gemini cationic liposomes was much better than their mono-head counterparts (designated as M12, M14, M16 and M18, respectively). In the same series of liposomes, the bonding ability declined with an increase in the tail length. They were tested for their gene-transferring capabilities in Hep-2 and A549 cells. They showed a higher transfection efficiency than their mono-head counterparts and were comparable or superior in transfection efficiency and cytotoxicity to the commercial liposomes, DOTAP and Lipofectamine 2000. Our results convincingly demonstrate that the gene-transferring capabilities of these cationic lipids depended on the hydrocarbon chain length. Gene transfection efficiency was maximal at a chain length of 14, as G14 can silence about 80% of luciferase in A549 cells. Cell uptake results indicate that Gemini lipid delivery systems could be internalised by cells very efficiently. Thus, the Gemini cationic lipids could be used as synthetic non-viral gene delivery carriers for further studies.