Hits:

Indexed by:期刊论文

Date of Publication:2009-07-01

Journal:JOURNAL OF ENVIRONMENTAL SCIENCES

Included Journals:SCIE、EI、PubMed、ISTIC、CSCD、Scopus

Volume:21

Issue:4

Page Number:503-508

ISSN No.:1001-0742

Key Words:redox mediator; sulfonated azo dyes; Sphingomonas xenophaga; reductase

Abstract:The effects of various quinone compounds on the decolorization rates of sulfonated azo dyes by Sphingomonas xenophaga QYY were investigated. The results showed that anthraquinone-2-sulfonate (AQS) was the most effective redox mediator and AQS reduction was the rate-limited step of AQS-mediated decolorization of sulfonated azo dyes. Based on AQS biological toxicity tests, it was assumed that AQS might enter the cells and kill them. In the cytoplasmic extracts from strain QYY, AQS more effectively increased decolorization rates of sulfonated azo dyes than other quinone compounds. In addition, we found a NADH/FMN-dependent AQS reductase using nondenaturing polyacrylamide gel electrophoresis (Native-PAGE).