Quinone-mediated decolorization of sulfonated azo dyes by cells and cell extracts from Sphingomonas xenophaga
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Indexed by: Journal Article
Date of Publication: 2009-07-01
Journal: JOURNAL OF ENVIRONMENTAL SCIENCES
Included Journals: Scopus、CSCD、ISTIC、PubMed、EI、SCIE
Volume: 21
Issue: 4
Page Number: 503-508
ISSN: 1001-0742
Key Words: redox mediator; sulfonated azo dyes; Sphingomonas xenophaga; reductase
Abstract: The effects of various quinone compounds on the decolorization rates of sulfonated azo dyes by Sphingomonas xenophaga QYY were investigated. The results showed that anthraquinone-2-sulfonate (AQS) was the most effective redox mediator and AQS reduction was the rate-limited step of AQS-mediated decolorization of sulfonated azo dyes. Based on AQS biological toxicity tests, it was assumed that AQS might enter the cells and kill them. In the cytoplasmic extracts from strain QYY, AQS more effectively increased decolorization rates of sulfonated azo dyes than other quinone compounds. In addition, we found a NADH/FMN-dependent AQS reductase using nondenaturing polyacrylamide gel electrophoresis (Native-PAGE).