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人胸腺素α1基因在毕赤酵母中的分泌表达

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Indexed by:期刊论文

Date of Publication:2003-04-20

Journal:生物技术

Volume:13

Issue:2

Page Number:4-6

ISSN No.:1004-311X

Key Words:人胸腺素α1 分泌表达 毕赤酵母

Abstract:根据质粒pPIC9K中信号肽基因和质粒pPIC3.5K/hTα1-RP,构建表达质粒pPIC9K/S-hTα1-RP,通过电激法转化到毕赤酵母GS115菌株中.甲醇诱导表达融合蛋白,SDS-PAGE和Western blot结果证明,重组基因hTα1-RP在酵母中得到了表达.

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