Release Time:2019-03-10  Hits:

Indexed by: Journal Article

Date of Publication: 2002-11-28

Journal: 遗传

Included Journals: ISTIC、PKU

Volume: 24

Issue: 6

Page Number: 679-683

ISSN: 0253-9772

Key Words: 人胸腺素α1;融合蛋白;毕赤酵母

Abstract: 根据已知人胸腺素1(human Thymosin α1,hTα1)的氨基酸序列和毕赤酵母偏爱密码子,人工合成了hTα1基因.用合成的hTα1基因取代核糖体蛋白(ribosomal protein,RP)基因5′端的84个碱基,组成重组基因hTα1-RP,连接到质粒pPIC3.5K上,构建表达质粒pPIC3.5K/hTα1-RP.表达质粒用电激法转化到毕赤酵母GS115菌株中.甲醇诱导表达融合蛋白,SDS-PAGE和Western印迹结果证明,重组基因hTα1-RP在酵母中得到了表达,表达量约为25mg/L.