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Study on the Interaction between Nuclease P1 and CuCl2 (II) by Visible Absorption Spectra

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Indexed by:期刊论文

Date of Publication:2000-01-01

Journal:Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis

Included Journals:Scopus、PKU

Volume:20

Issue:3

Page Number:309-310

ISSN No.:10000593

Abstract:In this paper, we studied the directly interaction between nuclease Pl and CuCl2 in aqueous solution by visible absorption spectra. As a result, the zinc ion in the active site of nuclease Pl can be replaced by additional Cu(II). At the the same time, Cu(II) entered into the active site of enzyme forming the correspondent derivatives of "Cu(II)-Pl". The variety of pH value influenced the types of derivatives and the enzyme activity.

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