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Indexed by:期刊论文

Date of Publication:2000-01-01

Journal:Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis

Included Journals:Scopus、PKU

Volume:20

Issue:3

Page Number:307-

ISSN No.:10000593

Abstract:The effect of changing the amount of external added Co(tip)n and pH values on the interaction of Cu2Zn2 SOD with organic metal compound (Cobalt(II)-Tryptophane) have been studied by means of ICP, VIS and measurement of enzyme activity. It has found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in the metalloenzyme (Cu2 Zn2SOD) with external added Co(Trp )n. As a result, part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives (Co(II)-substituted derivatives of SOD) were produced and the catalytic activity of enzyme were affected. It has also studied the intensity of interactions in different molar ratios (Cobalt(II)-Tryptophane) and pH values and got some results under the effect of corresponding factors.