Release Time:2019-03-11  Hits:

Indexed by: Journal Article

Date of Publication: 2000-01-01

Journal: Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis

Included Journals: PKU、Scopus

Volume: 20

Issue: 3

Page Number: 307-

ISSN: 10000593

Abstract: The effect of changing the amount of external added Co(tip)n and pH values on the interaction of Cu2Zn2 SOD with organic metal compound (Cobalt(II)-Tryptophane) have been studied by means of ICP, VIS and measurement of enzyme activity. It has found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in the metalloenzyme (Cu2 Zn2SOD) with external added Co(Trp )n. As a result, part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives (Co(II)-substituted derivatives of SOD) were produced and the catalytic activity of enzyme were affected. It has also studied the intensity of interactions in different molar ratios (Cobalt(II)-Tryptophane) and pH values and got some results under the effect of corresponding factors.