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Indexed by:期刊论文

Date of Publication:2001-01-01

Journal:Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis

Included Journals:Scopus、PKU

Volume:21

Issue:4

Page Number:433-434

ISSN No.:10000593

Abstract:In this paper, the results of EPR, VIS and enzyme activity show that alkaline phophatase from calf intest directly interacted with additional Cu( II ). The added Cu( II ) enter into A site of active sites of AKP which decrease enzyme activity. With increase of pH value, the decrease in the intensity of the Cu( II ) EPR signal and the change of place signal in VIS were observed. These results suggest that Cu( II ) migrate in the active center of the enzyme.