Study on the Interaction between Nuclease P1 and CuCl2 (II) by Visible Absorption Spectra
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论文类型：期刊论文
发表时间：2000-01-01
发表刊物：Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis
收录刊物：PKU、Scopus
卷号：20
期号：3
页面范围：309-310
ISSN号：10000593
摘要：In this paper, we studied the directly interaction between nuclease Pl and CuCl2 in aqueous solution by visible absorption spectra. As a result, the zinc ion in the active site of nuclease Pl can be replaced by additional Cu(II). At the the same time, Cu(II) entered into the active site of enzyme forming the correspondent derivatives of "Cu(II)-Pl". The variety of pH value influenced the types of derivatives and the enzyme activity.