点击次数：

论文类型：期刊论文

发表时间：2000-01-01

发表刊物：Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis

收录刊物：Scopus、PKU

卷号：20

期号：3

页面范围：307-

ISSN号：10000593

摘要：The effect of changing the amount of external added Co(tip)n and pH values on the interaction of Cu2Zn2 SOD with organic metal compound (Cobalt(II)-Tryptophane) have been studied by means of ICP, VIS and measurement of enzyme activity. It has found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in the metalloenzyme (Cu2 Zn2SOD) with external added Co(Trp )n. As a result, part of the metal ions in metalloenzyme were replaced and the corresponding metalloenzyme derivatives (Co(II)-substituted derivatives of SOD) were produced and the catalytic activity of enzyme were affected. It has also studied the intensity of interactions in different molar ratios (Cobalt(II)-Tryptophane) and pH values and got some results under the effect of corresponding factors.