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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：男
毕业院校：东北师范大学
学位：博士
所在单位：生物工程学院
电子邮箱：bioeng@dlut.edu.cn
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Study on the Direct Interaction between Alkaline Phophatase and Cu( II ) Ions by Spectral Analysis
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论文类型：期刊论文
发表时间：2001-01-01
发表刊物：Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis
收录刊物：Scopus、PKU
卷号：21
期号：4
页面范围：433-434
ISSN号：10000593
摘要：In this paper, the results of EPR, VIS and enzyme activity show that alkaline phophatase from calf intest directly interacted with additional Cu( II ). The added Cu( II ) enter into A site of active sites of AKP which decrease enzyme activity. With increase of pH value, the decrease in the intensity of the Cu( II ) EPR signal and the change of place signal in VIS were observed. These results suggest that Cu( II ) migrate in the active center of the enzyme.