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獐茅AIHAK1 Real-time PCR定量方法的建立

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Indexed by:期刊论文

Date of Publication:2009-06-20

Journal:中国农学通报

Included Journals:PKU、ISTIC

Volume:25

Issue:12

Page Number:20-24

ISSN No.:1000-6850

Key Words:獐茅;AIHAK1;real-time PCR;mRNA

Abstract:以β-actin为内参基因,根据已克隆的AIHAK1及β-actin碱基序列,分别设计了实时定量特异性引物,优化了反应的退火温度与引物浓度,并以优化的条件建立了相对定量标准曲线,同时对该方法的稳定性进行了评价.结果表明AIHAK1及β-actin基因的real-time PCR扩增效率分别为1.09和1.04,线性相关系数分别为0.996和0.997,批内及批间变异系数<5%.所建立的A1HAK1 real-time PCR定量方法操作简便、定量准确、重复性好,为进一步探索AIHAK1的功能、mRNA表达水平的变化及转AIHAK1农作物的检测奠定了方法学基础.

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