Release Time:2019-03-10  Hits:

Indexed by: Journal Article

Date of Publication: 2007-07-15

Journal: 植物学通报

Included Journals: CSCD、ISTIC、PKU

Volume: 24

Issue: 4

Page Number: 459-464

ISSN: 1003-2266

Key Words: 纤维素;辅酶A连接酶;4-香豆酸;木质素;尿苷二磷酸葡萄糖焦磷酸化酶;双价基因

Abstract: 构建了携带紫穗槐尿苷二磷酸葡萄糖焦磷酸化酶(UDP-glucose pyrophosphorylase,UGPase)基因和4-香豆酸:辅酶A连接酶(4-coumarate:coenzyme A ligase,4CL)反义基因的双价基因植物表达载体,用热激法转化至根癌农杆菌LBA4404中,并用制备的农杆菌工程菌进行了烟草转化.PCR及Southern杂交结果证实,双价基因已成功整合到烟草基因组中.综纤维素和硫酸木质素含量测定结果显示,转基因烟草纤维素含量增加,木质素含量减少,表明双价基因能够有效表达.