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个人信息Personal Information
副教授
硕士生导师
性别：女
毕业院校：东北林业大学
学位：博士
所在单位：生物工程学院
学科：植物学. 生物化学与分子生物学. 作物遗传育种
电子邮箱：dsuqiao@dlut.edu.cn
A choline monooxygenase gene promoter from Salicornia europaea increases expression of the beta-glucuronidase gene under abiotic stresses in tobacco (Nicotiana tabacum L.)
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论文类型：期刊论文
发表时间：2011-06-01
发表刊物：INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
收录刊物：Scopus、SCIE、PubMed
卷号：48
期号：3
页面范围：170-174
ISSN号：0301-1208
关键字：Abiotic stress; Choline monooxygenase; beta-Glucuronidase activity; Promoter Salicornia europaea
摘要：A 1312 bp 5' flanking region of Salicornia europaea choline monooxygenase (SeCMO) gene was isolated using the anchored PCR. To investigate the mechanism of regulation for this stress-induced gene, the SeCMO promoter-beta-glucuronidase (GUS) chimeric gene constructs containing five deletions F1, F2, F3, F4 and F5 were introduced into tobacco (Nicotiana tabacum L.) by Agrobacterium-mediated transformation. The functional properties of each promoter fragment were examined by assaying GUS activity in the leaves of transgenic tobacco treated with abiotic stresses (NaCl, PEG6000 and low temperature). The GUS activity in transgenic tobacco with F2 (-1056 to +8) construct showed highest increase under all the three abiotic stresses. Thus, the study provided a potential promoter induced by the salt, dehydration and cold for the plant genetic manipulation.