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Indexed by:期刊论文

Date of Publication:2018-08-21

Journal:ANALYTICAL CHEMISTRY

Included Journals:PubMed、SCIE

Volume:90

Issue:16

Page Number:9921-9928

ISSN No.:0003-2700

Abstract:Bacterial gamma-glutamyltranspeptidases (gamma-GT) is a well-known metabolic enzyme, which could cleave the gamma-glutamyl amide bond of gamma-glutamyl analogues. As a key metabolic enzyme of bacteria and a virulence factor for the host, bacterial gamma-GT was determined to be a novel pharmaceutical target for new antibiotics development. However, there is no efficient method for the sensing of gamma-GT activity in bacteria and the recognition of gamma-glutamyltransferase rich-bacteria. In the present work, a dicyanoisophorone derivative (ADMG) has been designed and developed to be a sensitive and selective near-infrared fluorescent probe for the sensing of bacterial gamma-GT. ADMG not only sensed bacterial gamma-GT in vitro, but also imaged intestinal bacteria in vivo. More interesting, the intestinal bacteria existed in the duodenum section of mouse displayed significant fluorescence emission. Under the guidance of the sensing of gamma-GT using ADMG, three intestinal bacteria strains K. pneumoniae CAV1042, K. pneumoniae XJRML-1, and E. faecalis were isolated successfully, which expressed the bacterial gamma-GT. Therefore, the fluorescent probe ADMG not only sensed the endogenous bacterial gamma-GT and imaged the intestinal bacteria but also guided the isolation of intestinal bacteria possessing gamma-GT efficiently, which suggested a novel biological tool for the rapid isolation of special bacteria from a mixed sample.