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Indexed by:期刊论文

Date of Publication:2014-12-15

Journal:SENSORS AND ACTUATORS B-CHEMICAL

Included Journals:SCIE、Scopus

Volume:205

Page Number:151-157

ISSN No.:0925-4005

Key Words:Human carboxylesterase 2; Ratiometric fluorescent probe; 4-Hydroxy-N-butyl-1,8-naphthalimide; Bioimaging

Abstract:A new ratiometric fluorescent probe derived from 4-hydroxy-N-butyl-1,8-naphthalimide (HNN) has been developed for selective detection of human carboxylesterase 2 (hCE2). The probe is designed by introducing benzoyl moiety to HNN, based on the intramolecular charge transfer (ICT) mechanism. The probe displays satisfying stability under physiological pH conditions with very low background fluorescence signal, but it can be rapidly hydrolyzed by hCE2 and release of HNN which leads to a remarkable red shift in emission spectra (148 nm). The newly designed probe exhibits excellent selectivity towards hCE2 over other human hydrolases, while the interference from various biologically relevant chemicals can be negligible. Its potential biological applications including inhibitor screening using human tissue preparations as enzyme sources, as well as fluorescence imaging of endogenous hCE2 in human living cells, have also been demonstrated. (C) 2014 Elsevier B.V. All rights reserved.