Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2013-12-01

Journal: DYES AND PIGMENTS

Included Journals: Scopus、EI、SCIE

Volume: 99

Issue: 3

Page Number: 537-542

ISSN: 0143-7208

Key Words: Two-photon fluorescence microscopy; Fluorescent probes; Hydrogen sulfide; Thiolysis; High selectivity; Cells imaging

Abstract: Fluorescent probes for hydrogen sulfide have received considerable attention because of the biological significance of H2S recognized recently. Two-photo microscopy offers advantages of increased penetration depth, localized excitation, and prolonged observation time. However, two-photon fluorescent probes for H2S are still rare. In this work, we introduced a dinitrophenyl ether group into the 4-position of 1,8-naphthalimide, which acts as the H2S reactive site, to efficiently yield compound NI-NHS as a two-photo fluorescent probe for H2S. The probe NI-NHS has a high selectivity for H2S over competitive anions and sulfide-containing analytes. This probe exhibits turn-on fluorescence detection of H2S in bovine serum and two-photon fluorescent imaging of H2S in living cells. (C) 2013 Elsevier Ltd. All rights reserved.