解磷细菌的筛选、双抗药性突变及培养
Release Time:2019-03-11 Hits:
Indexed by: Conference Paper
Date of Publication: 2009-05-10
Page Number: 1
Key Words: 解磷细菌;筛选;双抗药性;培养
Abstract: 筛选高效稳定的解磷菌株,并用氯霉素和氨苄青霉素进行双抗药性突变选育,为研究解磷菌在土壤中的定殖规律及根际适应性提供一种方法。【方法】通过溶磷圈观察,从Ca_3(PO_4)_2固体培养基上分离筛选解磷细菌。选取溶磷圈/菌落直径比较大、溶磷量较高且菌落形态有明显区别的代表性菌落,分别纯化5次以上,选择