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论文类型：期刊论文

发表时间：2008-03-01

发表刊物：TOXICOLOGY IN VITRO

收录刊物：SCIE、PubMed

卷号：22

期号：2

页面范围：430-437

ISSN号：0887-2333

关键字：protocatechuic acid; rotenone; apoptosis; mitochondrial dysfunction; PC12 cells

摘要：Protocatechuic acid (PCA), a phenolic compound isolated from the kernels of Alpinia (A.) oxyphylla, showed the significant neuroprotective effects on hydrogen peroxide (H(2)O(2)) or MPP(+)-induced apoptosis in cultured PC12 cells. However, the mechanism how PCA suppresses rotenone-induced neurotoxicity in cultured PC12 cells remains unclear. In this study, we investigated the protective effects of PCA in PC12 cells exposed to rotenone as an in vitro model of mitochondrial dysfunction and apoptotic cell death. The apoptosis in rotenone-induced PC12 cells was accompanied by the loss of mitochondrial membrane potential, the formation of reactive oxygen species (ROS), the total glutathione depletion, activation of caspase-3 and down-regulation of Bcl-2. In contrast, PCA markedly attenuated the above-mentioned mitochondrial dysfunction in a dose-dependent manner. Taken together, these results suggest that treatment of PC12 cells with PCA can block rotenone-induced apoptosis via ameliorating the mitochondrial dysfunction. (C) 2007 Elsevier Ltd. All rights reserved.