Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2013-07-01

Journal: BIORESOURCE TECHNOLOGY

Included Journals: PubMed、EI、SCIE

Volume: 140

Page Number: 15-21

ISSN: 0960-8524

Key Words: Biodegradation; Metabolites; Pseudomonas; Pyrene

Abstract: Using pyrene as a sole carbon, a new polycyclic aromatic hydrocarbons (PAHs)-degrading bacterial strain was isolated from the active sewage sludge. This strain was identified as Pseudomonas sp. Jpyr-1 by 16S rRNA gene sequence analysis. The maximum degradation rate of pyrene was 3.07 mg L-1 h(-1) in 48 h incubation with initial pyrene concentration of 200 mg L-1. Moreover, in binary system consisting of pyrene and another PAH, the enzyme system of Jpyr-1 showed a preference toward pyrene. Furthermore, competitive inhibition of pyrene degradation by other PAH compounds occurred in the binary system. Jpyr-1 could also rapidly degrade other PAHs, such as benzanthracene, chrysene and benzo[a]pyrene. Moreover, several metabolites were detected during pyrene degradation which indicated that Jpyr-1 degraded pyrene through the o-phthalate pathway. Taken together, these results indicated that Pseudomonas sp. Jpyr-1 was a new PAHs-degrading strain that might be useful in the bioremediation of sites contaminated with PAHs. (C) 2013 Elsevier Ltd. All rights reserved.