期刊论文

Ji, Qiuyan

Liu, GF (reprint author), Dalian Univ Technol, Sch Environm Sci & Technol, Minist Educ, Key Lab Ind Ecol & Environm Engn, Dalian 116024, Peoples R China.

Liu, Guangfei,Zhou, Jiti,Wang, Jing,Jin, Ruofei,Lv, Hong

2012-06-01

BIORESOURCE TECHNOLOGY

SCIE、EI、PubMed

J

114

144-148

0960-8524

Shewanella oneidensis MR-1; Water-insoluble dye; Sudan dye; Reduction

Decolorization of water-insoluble Sudan dyes was studied with Shewanella oneidensis MR-1, which removed 66.8%, 43.4%, 56.0% and 33.7% Sudan I-IV in 104 h, respectively and reduced Sudan I to aniline and 1-amino-2-naphthol. Lactate was identified as the most efficient electron donor for Sudan I reduction. Improved reduction performance was obtained in the presence of higher lactate or biomass concentration. The correlation between specific reduction rate and initial Sudan I concentration could be described with Michaelis-Menten kinetics (V-max = 1.8 mg Sudan I mg cell(-1) h(-1) and K-m = 5.3 mg l(-1)). The addition of anthraquinone-2-sulfonate stimulated the reduction significantly whereas the presence of 2-hydroxy-1,4-naphthoquinone had little enhancing effect. The main azoreductase activity was found with membrane-bound proteins of MR-1 and no reduction occurred when Sudan I was incubated with cell extracts. These data indicated for the first time that Shewanella could reduce solid-phase Sudan dye particles. (C) 2012 Elsevier Ltd. All rights reserved.