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异养硝化-好氧反硝化菌ADN-42的脱氮特性

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Indexed by:期刊论文

Date of Publication:2015-02-05

Journal:环境工程学报

Included Journals:PKU、ISTIC、CSCD、Scopus

Volume:9

Issue:2

Page Number:989-996

ISSN No.:1673-9108

Key Words:异养硝化 好氧反硝化 Pseudomonas sp.ADN-42 heterotrophic nitrification aerobic denitrification Pseudomonas sp.ADN-42

Abstract:从大连海域典型繁茂膜海绵(Hymeniacidon perleve)中筛选出1株耐盐异养硝化-好氧反硝化菌,通过形态观察、生理生化实验和16S rRNA基因序列分析,确定其为假单胞菌属(Pseudomonas),命名为ADN-42.其异养硝化-好氧反硝化条件为氯化铵为氮源,柠檬酸三钠为碳源,温度30℃,C/N值为12,摇床转速150 r/min,NH4+-N初始浓度约300 mg/L,盐度为40 g/L NaCl,在此条件下菌株84 h时NH4+-N去除率为75.4%,无硝态氮产生,亚硝态氮最大积累量为8.3 mg/L;将菌株投加到NH4+-N和NO2--N混合体系中,混合系统比仅以NH4+-N为氮源的体系的NH4+-N去除速率提高了12.7%;研究结果表明Pseudomonas sp.ADN-42可能是一株有着良好应用前景的高效耐盐异养硝化-好氧反硝化菌.

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