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论文类型：期刊论文

发表时间：2015-02-01

发表刊物：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录刊物：SCIE、EI、PubMed、Scopus

卷号：175

期号：4

页面范围：2000-2011

ISSN号：0273-2289

关键字：Pseudomonas sp ADN-42; Heterotrophic nitrification; Aerobic denitrification; N2O; DO

摘要：Recent research has highlighted the existence of some bacteria that are capable of performing heterotrophic nitrification and have a phenomenal ability to denitrify their nitrification products under aerobic conditions. A high-salinity-tolerant strain ADN-42 was isolated from Hymeniacidon perleve and found to display high heterotrophic ammonium removal capability. This strain was identified as Pseudomonas sp. via 16S rRNA gene sequence analysis. Gene cloning and sequencing analysis indicated that the bacterial genome contains N2O reductase function (nosZ) gene. NH3-N removal rate of ADN-42was very high. And the highest removal rate was 6.52 mg/L. h in the presence of 40 g/L NaCl. Under the condition of pure oxygen (DO > 8 mg/L), NH3-N removal efficiency was 56.9%. Moreover, 38.4% of oxygen remained in the upper gas space during 72 h without greenhouse gas N2O production. Keeping continuous and low level of dissolved oxygen (DO <3 mg/L) was helpful for better denitrification performance. All these results indicated that the strain has heterotrophic nitrification and aerobic denitrification abilities, which guarantee future application in wastewater treatment.