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Indexed by:期刊论文

Date of Publication:2016-04-01

Journal:JOURNAL OF FOOD AND DRUG ANALYSIS

Included Journals:SCIE、PubMed、Scopus

Volume:24

Issue:2

Page Number:399-405

ISSN No.:1021-9498

Key Words:Centrifugal partition chromatography; Content determination; Urea cream

Abstract:The objective of this study is to establish a centrifugal partition chromatography (CPC) method for determination of the urea ingredient in urea cream. The mechanism of this method is that urea is determined by UV detector at 430 nm after being extracted from the cream and derivatized on line via Ehrlich reaction in rotor of CPC, where the reaction products dissolve in the mobile phase and the cream matrix retains in the stationary phase. The mixed solvent consisting of n-hexane, methanol, hydrochloric acid and p-dimethylaminobenzaldehyde with a ratio of 1000 mL:1000 mL:18 mL:2.0 g is used for solvent system of CPC. The CPC method proposed offers good precision and convenience without complex sample pretreatment processes. Copyright (C) 2015, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.