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Indexed by:期刊论文

Date of Publication:2015-04-01

Journal:ELECTROPHORESIS

Included Journals:PubMed、SCIE、Scopus

Volume:36

Issue:7-8

Page Number:986-993

ISSN No.:0173-0835

Key Words:Beta-agonists; Chemiluminescence; Microflow injection analysis; Microfluidic chip

Abstract:-Agonists are a group of illegal feed additives. In this paper, it was found that the light emission produced by the oxidation of luminol by potassium ferricyanide was enhanced by the -agonists (ractopamine, salbutamol, and terbutaline). Based on chemiluminescence phenomenon, a novel, rapid, and sensitive microflow injection analysis system on a microfluidic glass chip was established for determination of the -agonists. The chip was fabricated from two glass plates (64 mmx32 mm) with microchannels of 200 m width and 100 m depth. The detection limits were achieved at 2.0x10(-8)mol/L of ractopamine, 1.0x10(-8)mol/L of terbutaline and 5.0x10(-7)mol/L of salbutamol. In this report, our method was applied for determination of the -agonists in swine hair from three different sources with satisfactory results.