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Indexed by:期刊论文

Date of Publication:2014-07-21

Journal:ANALYTICAL METHODS

Included Journals:SCIE、EI、Scopus

Volume:6

Issue:14

Page Number:5245-5249

ISSN No.:1759-9660

Abstract:The objective of this study was to establish a centrifugal partition chromatography (CPC) method for determination of the active ingredients in single-recipe ointments. The mechanism of this method was that the active ingredients were investigated using a UV detector after being extracted from the ointments by CPC, which worked as a fine separating funnel. Three ointments, containing salicylic acid, clotrimazole or aciclovir, were investigated using this method. The calibration curves of all three of the preparations were linear with a R-2 value higher than 0.998 in the tested concentration range, and the precision RSDs for salicylic acid, clotrimazole and aciclovir were 1.3%, 0.9% and 1.5%, respectively. Compared with the HPLC method, the CPC method had equivalent precision and accuracy, but could directly use the ointment solution as a sample instead of needing a complex sample preparation process. Furthermore, the CPC method also had several advantages in economic cost. In summary, the CPC method is a potential optimized technique to determine ointment formulations.