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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：男
毕业院校：日本富山医科药科大学
学位：博士
所在单位：化工学院
学科：药物化学. 药物分析学
办公地点：西部新校区G-302室（制药科学与技术学院）
联系方式：84986195
电子邮箱：zyzhao@dlut.edu.cn
Screening and identification of natural ligands of tyrosinase from Pueraria lobata Ohwi by a combination of ultrafiltration and LC-MS
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论文类型：期刊论文
发表时间：2017-09-07
发表刊物：ANALYTICAL METHODS
收录刊物：Scopus、SCIE、EI
卷号：9
期号：33
页面范围：4858-4862
ISSN号：1759-9660
摘要：Ultrafiltration combined with liquid chromatography-mass spectrometry (UF-LC-MS) is an efficient affinitybased method to screen potential ligands of biomacromolecules from natural sources. In order to investigate the effects of constituents from Pueraria labta Ohwi on tyrosinase, a UF-LC-MS method for screening tyrosinase ligands was established. Seven constituents were screened as tyrosinase ligands with high affinity and their structures were elucidated as isoflavones and isoflavone glycosides by mass spectrum analysis, among which puerarin exhibited much stronger inhibitory activity in vitro than arbutin against tyrosinase. An in silico docking study indicates that the interaction between puerarin and tyrosinase mainly derived from hydrogen bonds and pi-cation interaction.