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论文类型：期刊论文

发表时间：2013-06-21

发表刊物：ANALYTICAL METHODS

收录刊物：SCIE、EI、Scopus

卷号：5

期号：12

页面范围：3045-3049

ISSN号：1759-9660

摘要：In an attempt to establish the qualitative and quantitative analysis of Fructus ligustri lucidi, near-infrared spectroscopy (NIRS) was applied. The qualitative model can identify nine alcohol-broiled products from eight different geographical origins and one crude sample with a factorization algorithm and pretreatment of the second-derivative (2-Der). For the quantitative models, a partial least squares (PLS) algorithm with optimum parameters was applied for determination of oleanolic acid (OA) and ursolic acid (UA) in Fructus ligustri lucidi. There is no significant difference for the observed values between the NIRS and HPLC method since the results of the t-tests are 0.590 and 0.441, respectively, both less than t((0.05,19)) = 2.093 with a 95% confidence level. The data obtained revealed the proposed NIRS method is viable for the rapid qualitative and quantitative analysis of the active ingredients in Fructus ligustri lucidi.