个人信息Personal Information
教授
博士生导师
硕士生导师
性别:男
毕业院校:日本富山医科药科大学
学位:博士
所在单位:化工学院
学科:药物化学. 药物分析学
办公地点:西部新校区G-302室(制药科学与技术学院)
联系方式:84986195
电子邮箱:zyzhao@dlut.edu.cn
Rapid discrimination and quantification of alkaloids in Corydalis Tuber by near-infrared spectroscopy
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论文类型:期刊论文
发表时间:2012-02-05
发表刊物:JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
收录刊物:SCIE、PubMed、Scopus
卷号:59
期号:1
页面范围:44-49
ISSN号:0731-7085
关键字:Near-infrared spectroscopy classification; Quantification; Corydalis Tuber; Partial least squares regression
摘要:With the application of near-infrared spectroscopy (NIRS), a convenient and rapid method for determination of alkaloids in Corydalis Tuber extract and classification for samples from different locations have been developed. Five different samples were collected according to their geographical origin, 2-Der with smoothing point of 17 was applied as the spectral pre-treatment, and the 1st to scaling range algorithm was adjusted to be optimal approach, classification model was constructed over the wavelength range of 4582-4270 cm(-1), 5562-4976 cm(-1) and 7000-7467 cm(-1) with a great recognition rate. For prediction model, partial least squares (PLS) algorithm was utilized referring to HPLC-UV reference method, the optimum models were obtained after adjustment. Pre-processing methods of calibration models were COE for protopine and min-max normalization for palmatine and MSC for tetrahydropalmatine, respectively. The root mean square errors of cross-validation (RMSECV) for protopine, palmatine, tetrahydropalmatine were 0.884, 1.83, 3.23 mg/g. The correlation coefficients (R(2)) were 99.75, 98.41 and 97.34%. T test was applied, in the model of tetrahydropalmatine; there is no significant difference between NIR prediction and HPLC reference method at 95% confidence interval with t = 0.746 < t((0,05.20)) = 2.086, therefore NIRS is a reliable analytical tool in establishing prediction models. (C) 2011 Elsevier B.V. All rights reserved.