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Indexed by:期刊论文

Date of Publication:2022-06-30

Journal:微生物学杂志

Volume:38

Issue:1

Page Number:63-69

ISSN No.:1005-7021

Abstract:The conditions of nitrogen source in ferment medium for Lactobacillus pentosus was optimized. Different com-binations of nitrogen sources were optimized using xylose through single factors experiment and response surface analyses for lactic acid high yielding L. pentosus. The best combination of beef extract and ammonium citrate dibasic was obtained as followed:17.72 g/L of beef extract and 1.91 g/L of ammonium citrate dibasic,and practically produced 42.37 g/L of lactic acid at maximum. The best composite of inorganic nitrogen complex adding corn steep liquor and yeast powder, 46.54 g/L of corn steep liquor,21.95 g/L of yeast powder,and 9.95 g/L of ammonium citrate dibasic,could obtained 41.06 g/L of lactic acid at the maximum production. The kinds of organic nitrogen source were reduced through re-sponse surface optimization. The higher production of lactic acid was produced using the complex of beef extract and am-monium citrate dibasic,and reduced the amount of organic nitrogen sources, and saved the cost. The complex of corn steep liquor and ammonium citrate dibasic has solved the problem of the hyper-low utilization rate of xylose caused by u-sing corn steep liquor as single nitrogen source,still obtained higher concentration of lactic acid.

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