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Indexed by:期刊论文

Date of Publication:2012-03-01

Journal:PHYTOTHERAPY RESEARCH

Included Journals:SCIE、Scopus

Volume:26

Issue:3

Page Number:369-374

ISSN No.:0951-418X

Key Words:aucubin; hydrogen peroxide; apoptosis; oxidant-antioxidant balance; PC12 cells

Abstract:The present study investigated the neuroprotective effects of aucubin on hydrogen peroxide (H2O2)-induced apoptosis in PC12 cells. Exposure of PC12 cells to 0.25?mm H2O2 induced a leakage of lactate dehydrogenase and decreased cell viability, as shown by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. In a dose over 0.1?mm, aucubin increased PC12 cellular viability and markedly attenuated H2O2-induced apoptotic cell death. Quantitation of apoptosis by flow cytometry indicated that aucubin inhibited H2O2-induced apoptosis in PC12 cells. Nuclear damage was alleviated by aucubin, as shown by Hoechst staining. In addition, the levels of malondialdehyde were reduced and the activity of superoxide dismutase, catalase and glutathione peroxidase was augmented in these cells. These results indicated that aucubin inhibited H2O2-induced apoptosis in PC12 cells through regulation of the endogenous oxidantantioxidant balance. Our results suggest that aucubin is a potential protective agent for the treatment of oxidative-stress-induced neurodegenerative disease. Copyright (c) 2011 John Wiley & Sons, Ltd.