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Indexed by:期刊论文

Date of Publication:2011-06-01

Journal:MOLECULAR BIOLOGY REPORTS

Included Journals:SCIE

Volume:38

Issue:5

Page Number:3561-3567

ISSN No.:0301-4851

Key Words:Aucubin; Hydrogen peroxide; Apoptosis; PC12 cells; Bcl-2 family

Abstract:In this study, the effect of aucubin on H2O2-induced apoptosis was studied by using a rat pheochromocytoma (PC12) cell line. We have analyzed the apoptosis of H2O2-induced PC12 cells, H2O2-induced apoptosis appeared to correlate with lower Bcl-2 expression, higher Bax expression and sequential activation of caspase-3 leading to cleavage of poly-ADP-ribose polymerase (PARP). Aucubin not only inhibited lower Bcl-2 expression, high Bax expression, but also modulated caspase-3 activation, PARP cleavage, and eventually protected against H2O2-induced apoptosis. These results indicated that aucubin can obstruct H2O2-induced apoptosis by regulating of the expression of Bcl-2 and Bax, as well as suppression of caspases cascade activation.