Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2011-06-01

Journal: MOLECULAR BIOLOGY REPORTS

Included Journals: SCIE

Volume: 38

Issue: 5

Page Number: 3561-3567

ISSN: 0301-4851

Key Words: Aucubin; Hydrogen peroxide; Apoptosis; PC12 cells; Bcl-2 family

Abstract: In this study, the effect of aucubin on H2O2-induced apoptosis was studied by using a rat pheochromocytoma (PC12) cell line. We have analyzed the apoptosis of H2O2-induced PC12 cells, H2O2-induced apoptosis appeared to correlate with lower Bcl-2 expression, higher Bax expression and sequential activation of caspase-3 leading to cleavage of poly-ADP-ribose polymerase (PARP). Aucubin not only inhibited lower Bcl-2 expression, high Bax expression, but also modulated caspase-3 activation, PARP cleavage, and eventually protected against H2O2-induced apoptosis. These results indicated that aucubin can obstruct H2O2-induced apoptosis by regulating of the expression of Bcl-2 and Bax, as well as suppression of caspases cascade activation.